Investigate the Biological and Prognostic Association of Annexin A2 in Nasopharyngeal Carcinoma

The immunosuppressive responses in nasopharyngeal carcinoma (NPC) are mediated by annexin A2 through interact with DC-SIGN on dendritic cells. Annexin A2(ANXA2) is up regulated in several cancer and is associated with metastasis. However, the oncogenic effect and the clinical significance of ANXA2 have not been fully investigated in NPC. The short hairpin RNA (shRNA) knockdown of ANXA2 was used to examine the cellular effect, RT-PCR, Western blotting, immunofluorescence (IF) were applied to determine molecular expression levels. Xenograft mouse tumors were studied in vitro, Immunohistochemistry (IHC) was performed on tissue sections. The overall goals of this proposal are to investigate the tumorous function and mechanism of ANXA2 in NPC. The specific aims are as follows: (1) whether ANXA2 regulates multiple malignant phenotypes and serves as a tumor progression marker in Nasopharyngeal carcinoma. (2) Study on the molecular mechanism of ANXA2 associated with EMT in Nasopharyngeal Carcinoma. (3) What are the differences between ANXA2 knockdown NPC and normal NPC in both chemotherapy and radiotherapy. (4) Study on the molecular mechanism of Annexin A2-S100A10 and MMPs associated multiple malignant phenotypes on NPC. By this study, we will clarify the tumorous function and mechanism of ANXA2 in the Nasopharyngeal carcinoma. Moreover, we proposed that the ANXA2 may be an Important prognosis marker for clinical therapies including both chemotherapy and radiotherapy.