Functional Evaluation of Cnidii Fructus and Osthole on Endothelial Progenitor Cells by Animal and Cell Models(2-1)

Project: A - Government Institutionc - Ministry of Health and Welfare

Description

Common Cnidium Fruit is a traditional Chinese medicine that used externally to kill worms and relieve itching. And internally, it was used mainly on warming the kidney. Osthole, a coumarin analogue, is the main active component in Common Cnidium Fruit with lots of biological activities, including anti-hypertension, anti-diabetes, anti-insulin resistance, anti-inflammation and etc. Recently, our screening result on the pure compound library of Chinese medicine showed that osthole could potentially enhance the angiogenesis activity of human endothelial progenitor cells (EPCs). By its multiple functions on the alleviation of the risk factors of atherosclerosis, it’s interesting that Common Cnidium Fruit may have the potency on modulating the function of EPCs in vivo. In this two-year project, we will emphasize on the in vivo activities of Common Cnidium Fruit by using mice model, including the number of circulating EPCs and the angiogenetic potency in normal and diet-induced obesity situation by hindlimb ischemic model. And the cellular model will perform at the same time for simply observation of the activities of ethanolic extracts, partition fractions or the osthole on EPCs in much pure enviroments. The PCR array will be used for extensively signaling pathway mining to get more information for healthcare and extend the applicaiotns for Common Cnidium Fruit.
StatusFinished
Effective start/end date1/17/1312/15/13

Keywords

  • Common Cnidium Fruit
  • Osthole
  • EPCs
  • Angiogenesis