This research is a two-year project. The goal of first year (103) is the fabrication of the scaffold of bio-medical composite material and studies on its physical-chemical and degradation properties. This research used the PCL (Poly(ε-caprolcatone)), progen (NaCl), and graphene powders (graphene, graphene oxide) for fabricating the scaffold by particulate leaching method. Then this study discusses the mechanical, physical, chemical and surface properties by test method and this study compares the differences of different powders doped scaffold. The test results reference for cell adhension, expansion and proliferation on cell culture. This year also discuses the weight loss and change of pH value by dgradation on scaffold of bio-medical composite material. The pilot study of this year has fabricated the scaffold of bio-medical composite material. The results appear that the PCL mixed graphene or graphene oxide, the property of scaffold of bio-medical composite material is from hydrophobic to hydrophilic. The goal of second year (104) is cell culture and animal test for scaffold of bio-medical composite material from the results of first year (103). This research uses the sterilization method to deal with the scaffold of bio-medical composite material on the clean room. Then this research uses the osteoblastoma cells (MG63) and human mesenchymal stem cells (hMSC) to culture on the scaffold of bio-medical composite material. This research uses the SEM, TEM and laser confocal microscope (LCMS) to observe the cell grow situation after 1 day, 3 days, 5 days and 7 days. On the other hand, in order to explore the properties of the materials of these physicians, this project will study the cell-attached after the distribution of actin. This research did the MTT assay and ALP assay for cell culture. The immediate plans to use two-color staining and fluorescence microscope of actin distribution in cells and the extracelluar matrix and cell membrance with the cell membrance to form a dynamic network of actin proactive role in process of cell can be used as bio-compatibility of materials of verification reference. Then this research takes the scaffold of bio-medical composite material with osteoblastoma cell to transplant in the animal (mouse). Finally, this research uses SEM, TEM and Laser confocal microscope to observe the cell grow situation of scaffold of bio-medical composite material from taking out animal body for two weeks, four weeks, six weeks and eight weeks. Degradation process in the body for the scaffold if there is inflammation of the generated class of osteoblastoma cells in the bracket attached to the case and the case for bone tissue reconstruction of observation and analysis, and make a discussion of bio-compatibility. Finally, this research used the micro-CT to observe the animal tissue by scanning, analysis the porosity and strength of scaffold of bio-medical composite material and the results could act as the reference for optimal design of bio-medical scaffolds.
|Effective start/end date||8/1/14 → 9/30/15|
- Scaffold of bio-medical composite material
- Physical-chemical properties
- Cell culture
- Animal test