現代牙科手術大量使用人工骨材來填補受損部位,而傳統骨材的研發多著重於適 應骨母細胞的貼附生長,然而牙齒幹細胞對於齒槽骨的修復也很重要。我們的興趣在 發展新一代骨材,其必須具備以下特性:(1) 對牙齒間質幹細胞具有趨化吸引性;(2) 適 合牙齒間質幹細胞及骨母細胞貼附生長;(3) 能誘導牙齒間質幹細胞及骨母細胞的骨 化。為達此目的,本計畫將篩選對牙髓幹細胞具有特殊吸引性的趨化激素,以及可促 進牙髓幹細胞及骨母細胞骨化的黃酮類化合物,另外選擇一可攜帶黃酮類藥物的載體 蛋白,再以水膠、奈米陶瓷混合組成膠態可塗抹的人工骨凝膠,做為細胞貼附生長的 支架。本研究在體外試驗中將評估初代培養的人類牙髓幹細胞及骨母細胞的反應,接 著進行動物試驗,以蘭嶼猪為動物模型,在其下顎骨植入此人工骨凝膠,以測試其對 骨組織修復的效果。本研究對於新一代牙科用人工骨的研發至為重要。

Project: A - Government Institutionb - Ministry of Science and Technology

Description

Artificial bone scaffolds are widely used in current oral surgeries. Traditional bone scaffolds are designed mainly for the attachment and growth of osteoblasts, however, dental stem cells are also important and should not be ignored in the healing of mandibular bone defect. This study is devoted to developing a novel bone graft that has two advanced properties: (1) chemoattractive to dental mesenchymal stem cells (DPSC); (2) suitable for the attachment and growth of DPSC and osteoblast; (3) osteogenic inducing for DPSC and osteoblast. For this purpose, several chemokines candidates will be screened for their ability to chemoattract DPSC. In addition, flavonoids that could promote osteogenesis of DPSC and human primary osteoblast are selected. The flexible ceramic hydrogel scaffold is constituted with hydrogel and nano-hydroxyapatite for the attachment and growth of cells. In in vitro study, the drug-containing ceramic hydrogel will be evaluated in DPSC cells and human primary osteoblasts. In addition, the swine animal model will be applied for the evaluation of this ceramic hydrogel in vivo in the healing of mandibular bone defect. This study is important for the development of an advanced artificial bone graft in the future.
StatusFinished
Effective start/end date8/1/137/31/14

Keywords

  • alveolar
  • dental stem cell
  • artificial bone substitute
  • chemokine
  • flavonoids
  • osteogenesis